Evaluation of RT-PCR and reverse line blot hybridization for detection and genotyping F+ RNA coliphages from estuarine waters and molluscan shellfish
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چکیده
منابع مشابه
Multiplex PCR and Reverse Line Blot Hybridization Assay (mPCR/RLB)
Multiplex PCR/Reverse Line Blot Hybridization assay allows the detection of up to 43 molecular targets in 43 samples using one multiplex PCR reaction followed by probe hybridization on a nylon membrane, which is re-usable. Probes are 5' amine modified to allow fixation to the membrane. Primers are 5' biotin modified which allows detection of hybridized PCR products using streptavidin-peroxidase...
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ژورنال
عنوان ژورنال: Journal of Applied Microbiology
سال: 2008
ISSN: 1364-5072,1365-2672
DOI: 10.1111/j.1365-2672.2007.03646.x